|
Amino
Acid and Peptide Analysis
|
|
UltraSep ESD AP
|
|
|
The
column is suitable for the separation of derivatives of amino acids (A) and
peptides (P). The common procedures are summarized in the following table. If
samples are not often analyzed, esp. DABSYLchloride shows advantages.
|
Comparison
of different procedures for derivatization
|
|
Procedure
|
Detection
|
Limit of detection
|
Remarks
|
|
DABS
|
Absorption
436 nm
|
<
1 pmol
|
Stable
derivatives; primary and secondary amino acids can be determined
|
|
OPA
|
Fluorescence
330/440 nm
|
<
1 pmol
|
Pro, Hypro
and Cys cannot be determined
|
|
FMOC
|
Fluorescence
265/315 nm
|
<
1 pmol
|
His and Cys will be detected only in
part
|
DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
OPA = o-Phthalaldehyde
FMOC = 9-Fluorenylmethoxycarbonylchloride
|
|
|
Examples of
Analyzed Compounds |
Analyzed in Application
|
Go to |
|
Alanine (Ala)
|
AA |
|
|
Arginine (Arg)
|
AA |
AA |
|
Aspartic
acid (Asp)
|
AA |
|
|
Cystine (Cys2)
|
AA |
EPHARM6 |
|
Glutamic
acid (Glu)
|
AA |
|
|
Glycine (Gly)
|
AA |
|
|
Glutathione
|
EPHARM6 |
|
|
Histidine (His)
|
AA |
|
|
Isoleucine (Ile)
|
AA |
|
|
Leucine (Leu)
|
AA |
|
|
Lysine (Lys)
|
AA |
|
|
Methionine (Met)
|
AA |
|
|
Ornithine (Orn)
|
AA |
|
|
Phenylalanine (Phe)
|
AA |
|
|
Proline (Pro)
|
AA |
|
|
Serine (Ser)
|
AA |
|
|
Threonine (Thr)
|
AA |
|
|
Tyrosine (Tyr)
|
AA |
|
|
Valine (Val)
|
AA |
|
| COLUMNS
and PRICES
(€) |
| |
Column size (mm) |
| 250 x 4 |
250 x 3 |
250 x 2 |
125 x 4 |
125 x 3 |
125 x 2 |
| UltraSep ESD AP |
360.00 |
340.00 |
355.00 |
320.00 |
295.00 |
290.00 |
| |
Size (mm)
|
|
| 10 x 4 |
10 x 3 |
10 x 2 |
Precolumn
cartridges
(Price per pack = 5 pieces) |
170.00 |
170.00 |
170.00 |
| |
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