Selectivity Pack TRIS
3 Selectivities with one set or with one
Selectivity Pack TRIS and Column TRIS
The selectivity of the separation column is the
most important optimization criterion of HPLC. Until now for the
most used RP chromatography there didn't exist any column set with
defined and sufficiently different selectivities enabling users to
optimize their separations goal-directed.
This gap was closed by the selectivity pack „TRIS SEPSERV“ (TRIS = TRI Selectivities), introduced
by SEPSERV on the Analytica 2002. This set is made of 3 columns with
different selectivities, each 150 x 3 mm. The price of the set is 500.- €
(30 % advantage).
The set contains:
1. An UltraSep ES RP18
column of Pharm type, with high coverage (upper corner of the
2. a (special) column UltraSep ES RP18 M500 with
a low coverage, exhibiting so called „Extended
Polar (Matrix) Selectivity“, where silanol groups become effective
(left corner of the triangle), and
3. a polar RP column
of the type UltraSep ES AMID H RP18PA with so called „Embedded Polar
(Amid) Selectivity“ (right corner of the triangle), where embedded
amid groups become effective and silanols are shielded
[see GIT 46 (2002) (1) 72].
In addition to the set the column „Column TRIS“
is offered for the first time by SEPSERV. The reason lies in the
Sometimes the optimum separation cannot be obtained with any column
of the set (corners of the triangle) because it needs a
selectivity along a side of the triangle or coordinates within
the triangle (for instance 352).
In this case, the optimum column can be easily calculated on the basis of the retention times
on the 3
single columns isocratically obtained under identical conditions.
With these retention times, the customer can order a "tailor made"
Column TRIS from SEPSERV.
Furthermore, one can combine optimizations of the packing and of the eluent with each other.
TRIS (3 columns)