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Polycyclic
Aromatic Hydrocarbons (PAH)
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UltraSep ES PAH
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Polycyclic
aromatic
hydrocarbons are very widespread pollutions in environment, and many
laboratories analyze them.
10 years after introduction, our PAH columns are still top products on the
market. They reveal simple
separation conditions, an excellent resolution (base line separation) of all compounds
listed by EPA and DIN as well as short times of
analysis. The separation succeeds already with a simple water/acetonitrile
gradient. Therefore, combined gradients or even ternary gradients are
not necessary. Furthermore, a perfect wave length switching (fluorescence detection) is
possible. Internal standards (e.
g. Anthracene-d10,
Dibenz(a,h)anthracene-d14 or 2-Methylchrysene can be positioned
within the chromatogram without difficulties.
The
often tested columns yield very reproducible results with constant
retention times for more than 3000 injections. They work without problems in the
temperature range of 20 – 40 °C.
in the EU according to the
European Food Safety Authority (EFSA) the PAHs of the SCF (Scientific
Committee on Food) list have to be analyzed in food, additionally to the PAHs
of the EPA (Environmental Protection Agency, USA) list (see application PAHEFSA).
Using precolumns a contamination of the analytical columns by the samples can be
avoided. We recommend our PAH Precolumn Cartridges.
Take
into account, please, that 1 mm columns can be used effectively only, if
the volume of the detector is below 1 µl. For optimal gradient separations not
only the increase of the gradient, but also the adjusted volume flow is of importance. We
have optimized the separation conditions for you. The parameters specified in
the reference chromatograms are related to high pressure gradients. If you are working
with a low pressure gradient, the conditions have to be adapted because of the
greater dwell time.
In
the case that you can only work isocratically, keep in mind the large
requirements of analysis time if higher aromatics are present. We
recommend for this case a step gradient, which allows the separation of 2 x 8 =
16 aromatics within approximately 30 minutes. (I) Acetonitrile/water = 55/45,
(II) Acetonitrile/water = 90/10.
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UltraSep ES PAH Quick Columns
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The
top quality packing UltraSep ES PAH is available also with a particle diameter
of 4 µm. Because of that the separation columns reach an effectivity of >100000
TP/m and the duration of analysis can be shortened to one third without
loss of quality. One advantage of the Quick Columns is a rapid adjustment of
the equilibrium after each analysis. Therefore, chromatographical runs can
follow immediately after each other.
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Examples of Analyzed Compounds |
Analyzed in Application
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Go to |
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Acenaphthene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAH1 |
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Acenaphthylene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHEFSA |
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Anthracene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAH2 |
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Benz(a)anthracene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Benzo(b)fluoranthene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAHSUPER |
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Benzo(j)fluoranthene |
PAHEFSA |
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Benzo(k)fluoranthene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAHQC1 |
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Benzo(g,h,i)perylene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Benzo(a)pyrene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAHQC2 |
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Benzo(e)pyrene
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PAH2,
PAHSUPER,
PAHQC1, PAHEFSA |
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Chrysene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
PAHEFSA |
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Cyclopenta(c,d)pyrene |
PAHEFSA |
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Dibenz(a,h)anthracene |
PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
HYDROXYPHENANTHRENES |
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Dibenzo(a,e)pyrene |
PAHEFSA |
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Dibenzo(a,h)pyrene |
PAHEFSA |
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Dibenzo(a,i)pyrene |
PAHEFSA |
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Dibenzo(a,l)pyrene |
PAHEFSA |
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Fluoranthene |
PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Fluorene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Hydroxyphenanthrenes |
HYDROXYPHENANTHRENES |
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Indeno(1,2,3-c,d)pyrene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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2-Methylchrysene
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PAHQC2,
PAHEFSA |
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5-Methylchrysene |
PAHEFSA |
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1-Methylnaphthalene
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PAH2,
PAHSUPER,
PAHQC1, PAHQC2, PAHEFSA |
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2-Methylnaphthalene
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PAH2,
PAHSUPER,
PAHQC1, PAHQC2, PAHEFSA |
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Monohydroxyphenanthrenes |
HYDROXYPHENANTHRENES |
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Naphthalene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Phenanthrene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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Pyrene
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PAH1,
PAH2, PAHSUPER, PAHQC1, PAHQC2, PAHEFSA |
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COLUMNS
and PRICES
(€) |
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Column size (mm) |
250 x 4
200 x 4 |
250 x 3 *)
200 x 3 |
250 x 2
200 x 2 |
150 x 4
125 x 4 |
150 x 3
125 x 3 |
150 x 2
125 x 2 |
UltraSep ES PAH |
350.00 |
335.00 |
360.00 |
330.00 |
315.00 |
340.00
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UltraSep ES PAH
SUPER |
125
x 3 mm only |
380.00 |
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Size (mm) |
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10 x 4 |
10 x 3 |
10 x 2 |
Precolumn
cartridges
(Price per pack = 5 pieces) |
170.00 |
170.00 |
170.00 |
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Column size (mm) |
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60 x 4 |
60 x 3 |
60 x 2 |
40 x 2 |
33 x
3 |
UltraSep
ES PAH QC |
390.00 |
380.00 |
390.00 |
370.00 |
360.00 |
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Size (mm) |
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5 x 4 |
5 x 3 |
5 x 2 |
Precolumn
cartridges QC
(Price per pack = 5 pieces) |
170.00 |
170.00 |
170.00 |
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*)
This column is recommended for working with a linear water/methanol
gradient. The quality of the chromatogram is the same as with acetonitrile (see
SEPSERV
Analytic News). The initial pressure is 130 bar, the time of
analysis amounts 25 minutes. The detailed conditions of analysis are
disclosed along with delivering the column. |
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